Protein Domain : IPR004543

Type:  Family Name:  Translation elongation factor EFG/EF2, archaeal
Description:  Translation elongation factors are responsible for two main processes during protein synthesis on the ribosome [, , ]. EF1A (or EF-Tu) is responsible for the selection and binding of the cognate aminoacyl-tRNA to the A-site (acceptor site) of the ribosome. EF2 (or EF-G) is responsible for the translocation of the peptidyl-tRNA from the A-site to the P-site (peptidyl-tRNA site) of the ribosome, thereby freeing the A-site for the next aminoacyl-tRNA to bind. Elongation factors are responsible for achieving accuracy of translation and both EF1A and EF2 are remarkably conserved throughout evolution.Elongation factor EF2 (EF-G) is a G-protein. It brings about the translocation of peptidyl-tRNA and mRNA through a ratchet-like mechanism: the binding of GTP-EF2 to the ribosome causes a counter-clockwise rotation in the small ribosomal subunit; the hydrolysis of GTP to GDP by EF2 and the subsequent release of EF2 causes a clockwise rotation of the small subunit back to the starting position [, ]. This twisting action destabilises tRNA-ribosome interactions, freeing the tRNA to translocate along the ribosome upon GTP-hydrolysis by EF2. EF2 binding also affects the entry and exit channel openings for the mRNA, widening it when bound to enable the mRNA to translocate along the ribosome.This entry represents archaeal EF2 proteins (also known as aEF2), which are more similar to eukaryotic EF2 than to bacterial EF2 (or EFG), both in sequence similarity and in sharing with eukaryotes the property of having a diphthamide (modified His) residue at a conserved position. The diphthamide can be ADP-ribosylated by diphtheria toxin in the presence of NAD. Short Name:  Transl_elong_EFG/EF2_arc
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0 Child Features

8 Contains

DB identifier Type Name
IPR020568 Domain Ribosomal protein S5 domain 2-type fold
IPR009022 Domain Elongation factor G, III-V domain
IPR000640 Domain Translation elongation factor EFG, V domain
IPR004161 Domain Translation elongation factor EFTu/EF1A, domain 2
IPR000795 Domain Transcription factor, GTP-binding domain
IPR009000 Domain Translation protein, beta-barrel domain
IPR005517 Domain Translation elongation factor EFG/EF2, domain IV
IPR014721 Domain Ribosomal protein S5 domain 2-type fold, subgroup

2 Cross Referencess

Identifier
TIGR00490
MF_00054_A

0 Found In

4 GO Annotations

GO Term Gene Name
GO:0003746 IPR004543
GO:0005525 IPR004543
GO:0006414 IPR004543
GO:0005737 IPR004543

4 Ontology Annotations

GO Term Gene Name
GO:0003746 IPR004543
GO:0005525 IPR004543
GO:0006414 IPR004543
GO:0005737 IPR004543

0 Parent Features

0 Proteins

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            12762009
            12762047
            12932732
            15922593
            12762045