Protein Domain : IPR007035

Type:  Family Name:  Peptidase M55, D-aminopeptidase
Description:  Metalloproteases are the most diverse of the four main types of protease, with more than 50 families identified to date. In these enzymes, a divalent cation, usually zinc, activates the water molecule. The metal ion is held in place by amino acid ligands, usually three in number. The known metal ligands are His, Glu, Asp or Lys and at least one other residue is required for catalysis, which may play an electrophillic role. Of the known metalloproteases, around half contain an HEXXH motif, which has been shown in crystallographic studies to form part of the metal-binding site []. The HEXXH motif is relatively common, but can be more stringently defined for metalloproteases as 'abXHEbbHbc', where 'a' is most often valine or threonine and forms part of the S1' subsite in thermolysin and neprilysin, 'b' is an uncharged residue, and 'c' a hydrophobic residue. Proline is never found in this site, possibly because it would break the helical structure adopted by this motif in metalloproteases [].This group of metallopeptidases belong to MEROPS peptidase family M55 (DppA aminopeptidase family, clan MN). The type example is Bacillus subtilisDppA, which is a binuclear zinc-dependent, D-specific aminopeptidase. The structure reveals that DppA is a new example of a self-compartmentalising protease, a family of proteolytic complexes. Proteasomes are the most extensively studied representatives of this family. The DppA enzyme is composed of identical 30 kDa subunits organised in a decamer with 52 point-group symmetry. A 20 A wide channel runs through the complex, giving access to a central chamber holding the active sites. The structure shows DppA to be a prototype of a new family of metalloaminopeptidases characterised by the SXDXEG key sequence []. The only known substrates are D-ala-D-ala and D-ala-gly-gly. Short Name:  Peptidase_M55
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0 Child Features

0 Contains

3 Cross Referencess

Identifier
PF04951
PIRSF015853
SSF63992

0 Found In

0 GO Annotation

0 Ontology Annotations

0 Parent Features

4 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Pp3s30_460V3.1.p PAC:32948553 Physcomitrium patens 3268  
HORVU1Hr1G092980.20 PAC:38487687 Hordeum vulgare 669  
Ibeam.5248s0002.1.p PAC:39763450 Iberis amara 145  
CepurR40.1G228500.1.p PAC:43007188 Ceratodon purpureus R40 124  

2 Publications

First Author Title Year Journal Volume Pages PubMed ID
            7674922
            11473256