Protein Domain : IPR004722

Type:  Family Name:  Dihydroorotase
Description:  Dihydroorotase belongs to MEROPS peptidase family M38 (clan MJ), and includes peptides classified as a non-peptidase homologues. DHOase catalyses the third step in the de novobiosynthesis of pyrimidine, the conversion of ureidosuccinic acid (N-carbamoyl-L-aspartate) into dihydroorotate. Dihydroorotase binds a zinc ion which is required for its catalytic activity [].In bacteria, DHOase is a dimer of identical chains of about 400 amino-acid residues (gene pyrC). In higher eukaryotes, DHOase is part of a large multi-functional protein known as 'rudimentary' in Drosophila melanogasterand CAD in mammals and which catalyzes the first three steps of pyrimidine biosynthesis []. The DHOase domain is located in the central part of this polyprotein. In yeasts, DHOase is encoded by a monofunctional protein (gene URA4). However, a defective DHOase domain [] is found in a multifunctional protein (gene URA2) that catalyzes the first two steps of pyrimidine biosynthesis.The comparison of DHOase sequences from various sources shows [] that there are two highly conserved regions. The first located in the N-terminal extremity contains two histidine residues suggested [] to be involved in binding the zinc ion. The second is found in the C-terminal part. Members of this family of proteins are predicted to adopt a TIM barrel fold [].Dihydroorotase 'multifunctional complex type' , in contrast to the homodimeric type of dihydroorotase found in Escherichia coli, tends to appear in a large multifunctional complex with aspartate transcarbamoylase. Homologous domains appear in multifunctional proteins of higher eukaryotes. In some species, including Pseudomonas putidaand Pseudomonas aeruginosa, this protein is inactive but is required as a non-catalytic subunit of aspartate transcarbamoylase (ATCase). In these species, a second, active dihydroorotase is also present. Short Name:  DHOase
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1 Child Features

DB identifier Type Name
IPR024403 Family Dihydroorotase-like, bacterial

3 Contains

DB identifier Type Name
IPR011059 Domain Metal-dependent hydrolase, composite domain
IPR006680 Domain Amidohydrolase-related
IPR002195 Conserved_site Dihydroorotase, conserved site

3 Cross Referencess

Identifier
TIGR00857
MF_00220_A
MF_00220_B

0 Found In

3 GO Annotations

GO Term Gene Name
GO:0004151 IPR004722
GO:0046872 IPR004722
GO:0006221 IPR004722

3 Ontology Annotations

GO Term Gene Name
GO:0004151 IPR004722
GO:0046872 IPR004722
GO:0006221 IPR004722

0 Parent Features

2 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Pp3s46_240V3.1.p PAC:32948241 Physcomitrium patens 5700  
Pp3s46_240V3.3.p PAC:32948244 Physcomitrium patens 678  

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            9144792
            8098212
            2570735
            1671037
            2897615