Protein Domain : IPR005758

Type:  Family Name:  UDP-N-acetylmuramate--L-alanine ligase
Description:  This entry represents UDP-N-acetylmuramate-alanine ligase (MurC). MurC is an essential, cytoplasmic peptidoglycan biosynthetic enzyme, catalyzes the ATP-dependent ligation of L-alanine (Ala) and UDP-N-acetylmuramic acid (UNAM) to form UDP-N-acetylmuramyl-L-alanine (UNAM-Ala). The enzyme is a nonribosomal peptide ligase which utilises ATP to form an amide bond between L-alanine and UNAM.1 Mechanistic studies on the Escherichia coliMurC enzyme using oxygen isotope analyses demonstrated that the enzyme-catalyzed reaction proceeds through an acyl phosphate UNAM intermediate prior to L-alanine addition.The bacterial cell wall provides strength and rigidity to counteract internal osmotic pressure, and protection against the environment. The peptidoglycan layer gives the cell wall its strength, and helps maintain the overall shape of the cell. The basic peptidoglycan structure of both Gram-positive and Gram-negative bacteria is comprised of a sheet of glycan chains connected by short cross-linking polypeptides. Biosynthesis of peptidoglycan is a multi-step (11-12 steps) process comprising three main stages:(1) formation of UDP-N-acetylmuramic acid (UDPMurNAc) from N-acetylglucosamine (GlcNAc).(2) addition of a short polypeptide chain to the UDPMurNAc.(3) addition of a second GlcNAc to the disaccharide-pentapeptide building block and transport of this unit through the cytoplasmic membrane and incorporation into the growing peptidoglycan layer.Stage two involves four key Mur ligase enzymes: MurC () [], MurD () [], MurE () [] and MurF () []. These four Mur ligases are responsible for the successive additions of L-alanine, D-glutamate, meso-diaminopimelate or L-lysine, and D-alanyl-D-alanine to UDP-N-acetylmuramic acid. All four Mur ligases are topologically similar to one another, even though they display low sequence identity. They are each composed of three domains: an N-terminal Rossmann-fold domain responsible for binding the UDPMurNAc substrate; a central domain (similar to ATP-binding domains of several ATPases and GTPases); and a C-terminal domain (similar to dihydrofolate reductase fold) that appears to be associated with binding the incoming amino acid. The conserved sequence motifs found in the four Mur enzymes also map to other members of the Mur ligase family, including folylpolyglutamate synthetase, cyanophycin synthetase and the capB enzyme from Bacillales []. Short Name:  UDP-N-AcMur_Ala_ligase_murC

0 Child Features

3 Contains

DB identifier Type Name
IPR004101 Domain Mur ligase, C-terminal
IPR013221 Domain Mur ligase, central
IPR000713 Domain Mur ligase, N-terminal catalytic domain

2 Cross Referencess

Identifier
TIGR01082
MF_00046

0 Found In

2 GO Annotations

GO Term Gene Name
GO:0008763 IPR005758
GO:0009252 IPR005758

2 Ontology Annotations

GO Term Gene Name
GO:0008763 IPR005758
GO:0009252 IPR005758

0 Parent Features

85 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
402370 D8QQE9 PAC:15403522 Selaginella moellendorffii 499  
408505 D8R8I6 PAC:15422480 Selaginella moellendorffii 521  
orange1.1g009293m PAC:18125420 Citrus sinensis 538  
orange1.1g009279m PAC:18125419 Citrus sinensis 538  
Ciclev10004723m V4RM21 PAC:20791280 Citrus clementina 527  
Ciclev10004632m V4U7N1 PAC:20791279 Citrus clementina 575  
Gorai.007G012800.1 A0A0D2SC94 PAC:26784097 Gossypium raimondii 601  
55468 C1MKU9 PAC:27342915 Micromonas pusilla CCMP1545 594  
Thecc1EG015246t1 A0A061G8D7 PAC:27447489 Theobroma cacao 619  
Migut.F00294.1.p PAC:28933373 Mimulus guttatus 571  
Araha.11756s0420.1.p PAC:28856463 Arabidopsis halleri 449  
Prupe.2G300700.1.p A0A251QPN3 PAC:32074221 Prunus persica 574  
Pp3c23_890V3.1.p Q3LHF7 PAC:32949122 Physcomitrium patens 615  
Pp3c23_890V3.2.p PAC:32949121 Physcomitrium patens 626  
Pp3c23_890V3.3.p Q3LHF7 PAC:32949123 Physcomitrium patens 615  
Mapoly0106s0042.1.p A0A2R6WDC1 PAC:33017298 Marchantia polymorpha 659  
Aqcoe7G024800.1.p A0A2G5E0D1 PAC:33072874 Aquilegia coerulea 562  
Brdisv1pangenome1009080m.p PAC:33659087 Brachypodium distachyon Pangenome 476  
Brdisv1pangenome1009418m.p PAC:33608256 Brachypodium distachyon Pangenome 593  
Brdisv1pangenome1010009m.p PAC:33635549 Brachypodium distachyon Pangenome 471  
Brdisv1pangenome1011228m.p PAC:33607674 Brachypodium distachyon Pangenome 445  
Brdisv1pangenome1011420m.p PAC:33658469 Brachypodium distachyon Pangenome 432  
Brdisv1BdTR11A1046356m.p PAC:35694733 Brachypodium distachyon BdTR11a 457  
Brdisv1BdTR11A1047775m.p PAC:35689084 Brachypodium distachyon BdTR11a 471  
Brdisv1BdTR11A1040506m.p PAC:35693601 Brachypodium distachyon BdTR11a 476  
Brdisv1BdTR11A1041205m.p PAC:35693110 Brachypodium distachyon BdTR11a 432  
Brdisv1BdTR11A1041129m.p PAC:35689009 Brachypodium distachyon BdTR11a 607  
Solyc03g007970.1.1 PAC:36136781 Solanum lycopersicum 552  
PGSC0003DMP400018002 PAC:37463003 Solanum tuberosum 577  
VIT_216s0050g01080.1 PAC:38033823 Vitis vinifera 572  

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            17139082
            17427948
            16595662
            16322581
            16934839