Protein Domain : IPR000573

Type:  Domain Name:  Aconitase A/isopropylmalate dehydratase small subunit, swivel domain
Description:  Aconitase (aconitate hydratase; ) is an iron-sulphur protein that contains a [4Fe-4S]-cluster and catalyses the interconversion of isocitrate and citrate via a cis-aconitate intermediate. Aconitase functions in both the TCA and glyoxylate cycles, however unlike the majority of iron-sulphur proteins that function as electron carriers, the [4Fe-4S]-cluster of aconitase reacts directly with an enzyme substrate. In eukaryotes there is a cytosolic form (cAcn) and a mitochondrial form (mAcn) of the enzyme. In bacteria there are also 2 forms, aconitase A (AcnA) and B (AcnB). Several aconitases are known to be multi-functional enzymes with a second non-catalytic, but essential function that arises when the cellular environment changes, such as when iron levels drop [, ]. Eukaryotic cAcn and mAcn, and bacterial AcnA have the same domain organisation, consisting of three N-terminal alpha/beta/alpha domains, a linker region, followed by a C-terminal 'swivel' domain with a beta/beta/alpha structure (1-2-3-linker-4), although mAcn is small than cAcn. However, bacterial AcnB has a different organisation: it contains an N-terminal HEAT-like domain, followed by the 'swivel' domain, then the three alpha/beta/alpha domains (HEAT-4-1-2-3) [].This entry represents the 'swivel' domain found at the C-terminal of eukaryotic mAcn, cAcn/IPR1 and IRP2, and bacterial AcnA. This domain has a three layer beta/beta/alpha structure, and in cytosolic Acn is known to rotate between the cAcn and IRP1 forms of the enzyme. This domain is also found in the small subunit of isopropylmalate dehydratase (LeuD).3-isopropylmalate dehydratase (or isopropylmalate isomerase; ) catalyses the stereo-specific isomerisation of 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. This enzyme performs the second step in the biosynthesis of leucine, and is present in most prokaryotes and many fungal species. The prokaryotic enzyme is a heterodimer composed of a large (LeuC) and small (LeuD) subunit, while the fungal form is a monomeric enzyme. Both forms of isopropylmalate are related and are part of the larger aconitase family []. Short Name:  AconitaseA/IPMdHydase_ssu_swvl
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0 Child Features

0 Contains

1 Cross References

Identifier
PF00694

10 Found Ins

DB identifier Type Name
IPR011827 Family Hydrolyase LeuD/HacB/DmdB
IPR015937 Family Aconitase/isopropylmalate dehydratase
IPR006249 Family Aconitate/Iron-responsive element-binding protein 2
IPR006248 Family Aconitase, mitochondrial-like
IPR004431 Family 3-isopropylmalate dehydratase, small subunit
IPR015934 Family Aconitase/2-methylisocitrate dehydratase
IPR012708 Family 2-methylisocitrate dehydratase AcnD, Fe/S-dependent
IPR004418 Family Homoaconitase, mitochondrial
IPR006250 Family Aconitase, putative
IPR011824 Family Hydrolyase LeuD/DmdB, bacterial

1 GO Annotation

GO Term Gene Name
GO:0008152 IPR000573

1 Ontology Annotations

GO Term Gene Name
GO:0008152 IPR000573

1 Parent Features

DB identifier Type Name
IPR015928 Domain Aconitase/3-isopropylmalate dehydratase, swivel

3256 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
443331 D8S0I5 PAC:15412963 Selaginella moellendorffii 949  
119930 D8SLS4 PAC:15415349 Selaginella moellendorffii 187  
132907 D8T624 PAC:15409123 Selaginella moellendorffii 907  
evm.model.supercontig_12.301 PAC:16406861 Carica papaya 806  
evm.model.supercontig_29.188 PAC:16416219 Carica papaya 256  
evm.model.supercontig_6.311 PAC:16423500 Carica papaya 891  
evm.model.supercontig_87.76 PAC:16427747 Carica papaya 996  
29784.m000362 B9SXB6 PAC:16808465 Ricinus communis 900  
27777.m000279 PAC:16799168 Ricinus communis 997  
29600.m000550 B9SDW5 PAC:16803491 Ricinus communis 997  
Cucsa.043950.2 PAC:16953124 Cucumis sativus 811  
Cucsa.043950.4 PAC:16953126 Cucumis sativus 797  
Cucsa.043950.3 PAC:16953125 Cucumis sativus 797  
Cucsa.043950.1 PAC:16953123 Cucumis sativus 900  
Cucsa.129780.1 PAC:16962336 Cucumis sativus 253  
Cucsa.148530.1 PAC:16964165 Cucumis sativus 989  
orange1.1g002785m A0A067FVC5 PAC:18098894 Citrus sinensis 881  
orange1.1g002610m A0A067G720 PAC:18098891 Citrus sinensis 900  
orange1.1g003763m A0A067G724 PAC:18098896 Citrus sinensis 797  
orange1.1g008433m A0A067FVD1 PAC:18098898 Citrus sinensis 565  
orange1.1g002630m A0A067FVH3 PAC:18098892 Citrus sinensis 898  
orange1.1g003617m A0A067G7H0 PAC:18098895 Citrus sinensis 807  
orange1.1g002715m A0A067FYY8 PAC:18098893 Citrus sinensis 889  
orange1.1g005323m A0A067FVH7 PAC:18098897 Citrus sinensis 702  
orange1.1g025777m A0A067F5I6 PAC:18094446 Citrus sinensis 248  
orange1.1g003793m A0A067FH23 PAC:18108596 Citrus sinensis 795  
orange1.1g001863m A0A067FHT6 PAC:18108594 Citrus sinensis 1002  
orange1.1g002829m A0A067F5Z2 PAC:18108595 Citrus sinensis 875  
orange1.1g002651m A0A067EHL2 PAC:18119428 Citrus sinensis 896  
orange1.1g002599m A0A067EL88 PAC:18119427 Citrus sinensis 902  

3 Publications

First Author Title Year Journal Volume Pages PubMed ID
            9020582
            10087914
            15877277