Protein Domain : IPR023970

Type:  Family Name:  Methylthiotransferase/radical SAM-type protein
Description:  This entry represents a superfamily of enzymes utilizing both a 4Fe4S cluster and S-adenosyl methionine (SAM) to initiate radical reactions.The methylthiotransferase (MTTase) or miaB-like family is named after the (dimethylallyl)adenosine tRNA MTTase miaB protein, which catalyses a C-H to C-S bond conversion in the methylthiolation of tRNA. A related bacterial enzyme rimO performs a similar methylthiolation, but on a protein substrate. RimO acts on the ribosomal protein S12 and forms a separate MTTase subfamily. The miaB-subfamily includes mammalian CDK5 regulatory subunit-associated proteins and similar proteins in other eukaryotes. Two other subfamilies, yqeV and CDKAL1, are named after a Bacillus subtilis and a human protein, respectively. While yqeV-like proteins are found in bacteria, CDKAL1 subfamily members occur in eukaryotes and in archaebacteria. The likely MTTases from these 4 subfamilies contain an N-terminal MTTase domain, a central radical generating fold and a C-terminal TRAM domain (see ). The core forms a radical SAM fold (or AdoMet radical), containing a cysteine motif CxxxCxxC that binds a [4Fe-4S] cluster [, , ]. A reducing equivalent from the [4Fe-4S]+ cluster is used to cleave S-adenosylmethionine (SAM) to generate methionine and a 5'-deoxyadenosyl radical. The latter is thought to produce a reactive substrate radical that is amenable to sulphur insertion [, ]. The N-terminal MTTase domain contains 3 cysteines that bind a second [4Fe-4S]cluster, in addition to the radical-generating [4Fe-4S] cluster, which could be involved in the thiolation reaction. The C-terminal TRAM domain is not shared with other radical SAM proteins outside the MTTase family. The TRAM domain can bind to RNA substrate and seems to be important for substrate recognition. The tertiary structure of the central radical SAM fold has six beta/alpha motifs resembling a three-quarter TIM barrel core (see ) []. The N-terminal MTTase domain might form an additional [beta/alpha]2 TIM barrel unit []. Short Name:  MeThioTfrase/rSAM
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13 Child Features

DB identifier Type Name
IPR005839 Family Methylthiotransferase
IPR023862 Family Conserved hypothetical protein CHP03960, radical SAM
IPR011772 Family Magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase
IPR017834 Family Hopanoid biosynthesis associated radical SAM protein HpnJ
IPR030837 Family Putative variant cofactor biosynthesis B12-binding domain/radical SAM domain protein 1
IPR030969 Family Tryptophan 2-C-methyltransferase
IPR027564 Family Hopanoid C-3 methylase HpnR
IPR027559 Family B12-binding domain/radical SAM domain protein, rhizo-twelve system
IPR023969 Family Conserved hypothetical protein CHP04072, B12-binding/radical SAM-type
IPR023980 Family Conserved hypothetical protein CHP04013, B12-binding/radical SAM-type
IPR023984 Family Ribosomal peptide maturation radical SAM protein 1
IPR031003 Family Radical SAM P-methyltransferase, PhpK family
IPR023979 Family Conserved hypothetical protein CHP04014, B12-binding/radical SAM-type

0 Contains

1 Cross References

Identifier
PTHR11918

0 Found In

0 GO Annotation

0 Ontology Annotations

0 Parent Features

887 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
31574 D8RF06 PAC:15417785 Selaginella moellendorffii 421  
8469 D8TBJ8 PAC:15405180 Selaginella moellendorffii 82  
137168 D8TD33 PAC:15417777 Selaginella moellendorffii 98  
180500 D8SKD9 PAC:15414005 Selaginella moellendorffii 469  
424054 D8SNN2 PAC:15421409 Selaginella moellendorffii 613  
424158 D8SP04 PAC:15422197 Selaginella moellendorffii 353  
8473 D8SNN1 PAC:15405195 Selaginella moellendorffii 89  
123663 D8SS17 PAC:15402635 Selaginella moellendorffii 68  
446559 D8SSE5 PAC:15423187 Selaginella moellendorffii 686  
446506 D8SS14 PAC:15423064 Selaginella moellendorffii 936  
8456 PAC:15405143 Selaginella moellendorffii 91  
8455 PAC:15405141 Selaginella moellendorffii 91  
evm.model.supercontig_6.187 PAC:16423361 Carica papaya 405  
evm.model.supercontig_66.10 PAC:16424542 Carica papaya 634  
30074.m001410 B9S747 PAC:16816971 Ricinus communis 630  
29634.m002060 B9S686 PAC:16804201 Ricinus communis 535  
Cucsa.184640.1 PAC:16967583 Cucumis sativus 521  
Cucsa.061610.1 PAC:16954908 Cucumis sativus 635  
orange1.1g041524m A0A067FUI9 PAC:18098745 Citrus sinensis 524  
orange1.1g006956m A0A067GWL8 PAC:18091348 Citrus sinensis 624  
orange1.1g006836m A0A067GZA4 PAC:18091347 Citrus sinensis 629  
orange1.1g007001m A0A067GWA9 PAC:18091349 Citrus sinensis 622  
orange1.1g010162m A0A067H8T6 PAC:18091350 Citrus sinensis 516  
orange1.1g013195m A0A067H8F9 PAC:18091352 Citrus sinensis 448  
orange1.1g012929m A0A067GWB4 PAC:18091351 Citrus sinensis 453  
orange1.1g019874m A0A067GWM3 PAC:18091353 Citrus sinensis 334  
AT4G36390.1 Q8H0V1 PAC:19645627 Arabidopsis thaliana 640  
AT1G72090.1 Q9C7H2 PAC:19658225 Arabidopsis thaliana 601  
Thhalv10024669m V4P269 PAC:20193657 Eutrema salsugineum 632  
Thhalv10025020m V4LS77 PAC:20193658 Eutrema salsugineum 491  

4 Publications

First Author Title Year Journal Volume Pages PubMed ID
            18252828
            11882645
            11222759
            15289575