Protein Domain : IPR013848

Type:  Domain Name:  Methylthiotransferase, N-terminal
Description:  The methylthiotransferase (MTTase) or miaB-like family is named after the (dimethylallyl)adenosine tRNA MTTase miaB protein, which catalyses a C-H to C-S bond conversion in the methylthiolation of tRNA. A related bacterial enzyme rimO performs a similar methylthiolation, but on a protein substrate. RimO acts on the ribosomal protein S12 and forms a separate MTTase subfamily. The miaB-subfamily includes mammalian CDK5 regulatory subunit-associated proteins and similar proteins in other eukaryotes. Two other subfamilies, yqeV and CDKAL1, are named after a Bacillus subtilis and a human protein, respectively. While yqeV-like proteins are found in bacteria, CDKAL1 subfamily members occur in eukaryotes and in archaebacteria. The likely MTTases from these 4 subfamilies contain an N-terminal MTTase domain, a central radical generating fold and a C-terminal TRAM domain (see ). The core forms a radical SAM fold (or AdoMet radical), containing a cysteine motif CxxxCxxC that binds a [4Fe-4S] cluster [, , ]. A reducing equivalent from the [4Fe-4S]+ cluster is used to cleave S-adenosylmethionine (SAM) to generate methionine and a 5'-deoxyadenosyl radical. The latter is thought to produce a reactive substrate radical that is amenable to sulphur insertion [, ]. The N-terminal MTTase domain contains 3 cysteines that bind a second [4Fe-4S]cluster, in addition to the radical-generating [4Fe-4S] cluster, which could be involved in the thiolation reaction. The C-terminal TRAM domain is not shared with other radical SAM proteins outside the MTTase family. The TRAM domain can bind to RNA substrate and seems to be important for substrate recognition. The tertiary structure of the central radical SAM fold has six beta/alpha motifs resembling a three-quarter TIM barrel core (see ) []. The N-terminal MTTase domain might form an additional [beta/alpha]2 TIM barrel unit []. Short Name:  Methylthiotransferase_N

0 Child Features

0 Contains

2 Cross Referencess

Identifier
PF00919
PS51449

5 Found Ins

DB identifier Type Name
IPR005839 Family Methylthiotransferase
IPR006466 Family MiaB-like tRNA modifying enzyme, archaeal-type
IPR005840 Family Ribosomal protein S12 methylthiotransferase RimO
IPR006463 Family tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB
IPR006467 Family MiaB-like tRNA modifying enzyme

3 GO Annotations

GO Term Gene Name
GO:0003824 IPR013848
GO:0051539 IPR013848
GO:0009451 IPR013848

3 Ontology Annotations

GO Term Gene Name
GO:0003824 IPR013848
GO:0051539 IPR013848
GO:0009451 IPR013848

0 Parent Features

811 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
31574 D8RF06 PAC:15417785 Selaginella moellendorffii 421  
180500 D8SKD9 PAC:15414005 Selaginella moellendorffii 469  
evm.model.supercontig_66.10 PAC:16424542 Carica papaya 634  
30074.m001410 B9S747 PAC:16816971 Ricinus communis 630  
29634.m002060 B9S686 PAC:16804201 Ricinus communis 535  
Cucsa.368940.1 PAC:16981721 Cucumis sativus 1004  
Cucsa.184640.1 PAC:16967583 Cucumis sativus 521  
Cucsa.061610.1 PAC:16954908 Cucumis sativus 635  
orange1.1g041524m A0A067FUI9 PAC:18098745 Citrus sinensis 524  
orange1.1g006956m A0A067GWL8 PAC:18091348 Citrus sinensis 624  
orange1.1g006836m A0A067GZA4 PAC:18091347 Citrus sinensis 629  
orange1.1g007001m A0A067GWA9 PAC:18091349 Citrus sinensis 622  
orange1.1g010162m A0A067H8T6 PAC:18091350 Citrus sinensis 516  
orange1.1g013195m A0A067H8F9 PAC:18091352 Citrus sinensis 448  
orange1.1g012929m A0A067GWB4 PAC:18091351 Citrus sinensis 453  
orange1.1g019874m A0A067GWM3 PAC:18091353 Citrus sinensis 334  
AT4G36390.1 Q8H0V1 PAC:19645627 Arabidopsis thaliana 640  
AT1G72090.1 Q9C7H2 PAC:19658225 Arabidopsis thaliana 601  
Thhalv10024669m V4P269 PAC:20193657 Eutrema salsugineum 632  
Thhalv10025020m V4LS77 PAC:20193658 Eutrema salsugineum 491  
Thhalv10000077m V4M2D4 PAC:20208263 Eutrema salsugineum 647  
Thhalv10000140m V4L7P6 PAC:20208264 Eutrema salsugineum 500  
Thhalv10000144m V4LRD8 PAC:20208265 Eutrema salsugineum 493  
Thhalv10018307m V4KAB8 PAC:20192110 Eutrema salsugineum 603  
Ciclev10000888m V4T416 PAC:20785912 Citrus clementina 516  
Ciclev10001135m V4SJM2 PAC:20785913 Citrus clementina 448  
Ciclev10000582m V4V758 PAC:20785911 Citrus clementina 629  
Ciclev10001774m V4TE28 PAC:20785914 Citrus clementina 335  
Ciclev10026963m V4SP27 PAC:20799358 Citrus clementina 610  
Lus10016926 PAC:23159604 Linum usitatissimum 610  

4 Publications

First Author Title Year Journal Volume Pages PubMed ID
            18252828
            11882645
            11222759
            15289575