Protein Domain : IPR006358

Type:  Family Name:  Transcription elongation factor GreB
Description:  Bacterial GreA and GreB promote transcription elongation by stimulating an endogenous, endonucleolytic transcript cleavage activity of the RNA polymerase, allowing RNA transcription to continue past template-encoded arresting sites. GreA and GreB are sequence homologues and have homologues inevery known bacterial genome. GreA and GreB stimulate transcript cleavage in different ways; GreA induces cleavage of 3'-RNA fragments 2-3 nt in length and can only preventthe formation of arrested complexes, whereas GreB induces cleavage of fragments up to 18 nt in length and can rescue preexisting arrested complexes [].A 15 Angstrom resolution helical reconstruction of the Escherichia colicore RNA polymerase (RNAP)/GreB complex that allows fitting of high-resolution RNAP and GreB structures. The model of the complex reveals a remarkable binding mode for GreB; the globular C-terminal domain binds RNAP at the edge of the active site channel, while the N-terminal coiled-coil domain extends 45 Angstrom into a channel directly to the RNAP active site. The results point to a key role for conserved acidic residues at the tip of the Gre factor coiled coil in modifying the RNAP active site to catalyse the transcript cleavage reaction, and mutational studies confirm that these positions are critical for Gre factor function. Functional differences between GreA and GreB correlate with the distribution of positively charged residues on one face of the N-terminal coiledcoil. Short Name:  Tscrpt_elong_fac_GreB
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0 Child Features

3 Contains

DB identifier Type Name
IPR022691 Domain Transcription elongation factor, GreA/GreB, N-terminal
IPR001437 Domain Transcription elongation factor, GreA/GreB, C-terminal
IPR018151 Conserved_site Transcription elongation factor, GreA/GreB, conserved site

2 Cross Referencess

Identifier
TIGR01461
MF_00930

0 Found In

2 GO Annotations

GO Term Gene Name
GO:0003677 IPR006358
GO:0032784 IPR006358

2 Ontology Annotations

GO Term Gene Name
GO:0003677 IPR006358
GO:0032784 IPR006358

1 Parent Features

DB identifier Type Name
IPR028624 Family Transcription elongation factor GreA/GreB

4 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Brdisv1pangenome1006576m.p PAC:33623764 Brachypodium distachyon Pangenome 927  
Brdisv1pangenome1006793m.p PAC:33655608 Brachypodium distachyon Pangenome 906  
Brdisv1BdTR11A1041598m.p PAC:35689264 Brachypodium distachyon BdTR11a 906  
Brdisv1BdTR11A1041383m.p PAC:35696436 Brachypodium distachyon BdTR11a 927  

1 Publications

First Author Title Year Journal Volume Pages PubMed ID
            12914698