Protein Domain : IPR004430

Type:  Family Name:  3-isopropylmalate dehydratase, large subunit
Description:  3-isopropylmalate dehydratase (or isopropylmalate isomerase; ) catalyses the stereo-specific isomerisation of 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. This enzyme performs the second step in the biosynthesis of leucine, and is present in most prokaryotes and many fungal species. The prokaryotic enzyme is a heterodimer composed of a large (LeuC) and small (LeuD) subunit, while the fungal form is a monomeric enzyme. Both forms of isopropylmalate are related and are part of the larger aconitase family []. Aconitases are mostly monomeric proteins which share four domains in common and contain a single, labile [4Fe-4S]cluster. Three structural domains (1, 2 and 3) are tightly packed around the iron-sulphur cluster, while a fourth domain (4) forms a deep active-site cleft. The prokaryotic enzyme is encoded by two adjacent genes, leuC and leuD, corresponding to aconitase domains 1-3 and 4 respectively [, ]. LeuC does not bind an iron-sulphur cluster. It is thought that some prokaryotic isopropylamalate dehydrogenases can also function as homoaconitase , converting cis-homoaconitate to homoisocitric acid in lysine biosynthesis []. Homoaconitase has been identified in higher fungi (mitochondria) and several archaea and one thermophilic species of bacteria, Thermus thermophilus[]. This entry represents the large subunit of 3-isopropylmalate dehydratase, or the large subunit domain of single-chain forms of this enzyme. Homoaconitase, aconitase, and 3-isopropylmalate dehydratase have similar overall structures. All are dehydratases () and bind a [4Fe-4S]-cluster. 3-isopropylmalate dehydratase is split into large (leuC) and small (leuD) chains in eubacteria. Several pairs of archaeal proteins resemble the leuC and leuD pair in length and sequence but even more closely resemble the respective domains of homoaconitase, and their identity is uncertain. The archaeal leuC-like proteins are not included in group. Short Name:  3-IsopropMal_deHydase_lsu
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0 Child Features

4 Contains

DB identifier Type Name
IPR015931 Domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3
IPR001030 Domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
IPR015932 Domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2
IPR018136 Binding_site Aconitase family, 4Fe-4S cluster binding site

2 Cross Referencess

Identifier
TIGR00170
MF_01026

0 Found In

3 GO Annotations

GO Term Gene Name
GO:0003861 IPR004430
GO:0051539 IPR004430
GO:0009098 IPR004430

3 Ontology Annotations

GO Term Gene Name
GO:0003861 IPR004430
GO:0051539 IPR004430
GO:0009098 IPR004430

1 Parent Features

DB identifier Type Name
IPR015937 Family Aconitase/isopropylmalate dehydratase

22 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
196778 C1MKS1 PAC:27342776 Micromonas pusilla CCMP1545 770  
96879 C1FEI6 PAC:27398945 Micromonas sp RCC299 719  
28677 A4RQI6 PAC:27417859 Ostreococcus lucimarinus 722  
Brdisv1pangenome1001111m.p PAC:33641017 Brachypodium distachyon Pangenome 626  
Brdisv1pangenome1006840m.p PAC:33658316 Brachypodium distachyon Pangenome 649  
Brdisv1pangenome1006738m.p PAC:33658027 Brachypodium distachyon Pangenome 634  
Brdisv1pangenome1007825m.p PAC:33623887 Brachypodium distachyon Pangenome 470  
Brdisv1pangenome1008601m.p PAC:33659032 Brachypodium distachyon Pangenome 660  
Brdisv1pangenome1010237m.p PAC:33623998 Brachypodium distachyon Pangenome 468  
Brdisv1pangenome1011507m.p PAC:33656461 Brachypodium distachyon Pangenome 687  
Brdisv1BdTR8i1044410m.p PAC:34241092 Brachypodium distachyon BdTR8i 470  
Brdisv1BdTR11G1045553m.p PAC:34643465 Brachypodium distachyon BdTR11g 523  
Brdisv1BdTR11A1048113m.p PAC:35691552 Brachypodium distachyon BdTR11a 468  
Brdisv1BdTR11A1009822m.p PAC:35657541 Brachypodium distachyon BdTR11a 626  
Brdisv1BdTR11A1044500m.p PAC:35688049 Brachypodium distachyon BdTR11a 624  
Brdisv1BdTR11A1040058m.p PAC:35689421 Brachypodium distachyon BdTR11a 665  
Brdisv1BdTR11A1040513m.p PAC:35689985 Brachypodium distachyon BdTR11a 658  
Brdisv1BdTR11A1041247m.p PAC:35692853 Brachypodium distachyon BdTR11a 687  
Brdisv1BdTR11A1041633m.p PAC:35696844 Brachypodium distachyon BdTR11a 649  
Brdisv1BdTR11A1041541m.p PAC:35689071 Brachypodium distachyon BdTR11a 668  
Araha.58172s0006.1.p PAC:28856717 Arabidopsis halleri 428  
Araha.58172s0006.1.p PAC:28856717 Arabidopsis halleri 428  

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            9020582
            9813279
            15522288
            1400210
            16524361