Protein Domain : IPR006605

Type:  Domain Name:  G2 nidogen/fibulin G2F
Description:  Basement membranes are sheet-like extracellular matrices found at the basal surfaces of epithelia and condensed mesenchyma. By preventing cell mixing andproviding a cell-adhesive substrate, they play crucial roles in tissue development and function. Basement menbranes are composed of an evolutionarilyancient set of large glycoproteins, which includes members of the laminin family, collagen IV, perlecan and nidogen/entactin. Nidogen/entactin is animportant basement membrane component, which promotes cell attachment, neutrophil chemotaxis, trophoblast outgrowth, and angiogenesis. It consists ofthree globular regions, G1-G3. G1 and G2 are connected by a thread-like structure, whereas that between G2 and G3 is rod-like [, ].The nidogen G2 region binds to collagen IV and perlecan. The nidogen G2 structure is composed of two domains, an N-terminal EGF-like domain and a much larger beta-barrel domain of ~230 residues. The nidogen G2 beta-barrel consists of an 11-stranded beta-barrelof complex topology, the interior of which is traversed by the hydrophobic, predominantly alpha helical segment connecting strands C and D. The N-terminalhalf of the barrel comprises two beta-meanders (strands A-C and D-F) linked by the buried alpha-helical segment. The polypeptide chain then crosses thebottom of the barrel and forms a five-stranded Greek key motif in the C- terminal half of the domain. Helix alpha3 caps the top of the barrel and formsthe interface to the EGF-like domain. The nidogen G2 beta-barrel domain has unexpected structural similarity to green fluorescent protein, suggesting thatthey derive from a common ancestor. A large surface patch on the barrel surface is strikingly conserved in all metazoan nidogens. Site-directedmutagenesis demonstrates that the conserved residues in the conserved patch are involved in the binding of perlecan, and possibly also of collagen IV []. Short Name:  G2_nidogen/fibulin_G2F
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0 Child Features

0 Contains

3 Cross Referencess

Identifier
PF07474
PS50993
SM00682

0 Found In

0 GO Annotation

0 Ontology Annotations

1 Parent Features

DB identifier Type Name
IPR009017 Domain Green fluorescent protein

3 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Brara.I05065.1.p A0A397YC48 PAC:30643699 Brassica rapa FPsc 268  
Brara.I05065.2.p A0A397YC48 PAC:30643700 Brassica rapa FPsc 268  
Gomus.A07G011100.1.p A0A5D2YFG9 PAC:42038731 Gossypium mustelinum 33  

2 Publications

First Author Title Year Journal Volume Pages PubMed ID
            11427896
            9633511