Protein Domain : IPR017335

Type:  Family Name:  E3 ubiquitin-protein ligase RNF8
Description:  This entry represents E3 ubiquitin-protein ligase RNF8, which may be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. This enzyme promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer [, ]. Substrates that are poly-ubiquitinated at 'Lys-63' are usually not targeted for degradation. RNF8 acts following DNA double-strand break (DSB) formation, and is recruited to the sites of damage by ATM-phosphorylated MDC1, where it promotes the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF) [, ]. It may play a role in the regulation of RXRA-mediated transcriptional activity, but is not involved in RXRA ubiquitination by UBE2E2.Ubiquitinylation is an ATP-dependent process that involves the action of at least three enzymes: a ubiquitin-activating enzyme (E1, ), a ubiquitin-conjugating enzyme (E2, ), and a ubiquitin ligase (E3, , ), which work sequentially in a cascade. There are many different E3 ligases, which are responsible for the type of ubiquitin chain formed, the specificity of the target protein, and the regulation of the ubiquitinylation process []. Ubiquitinylation is an important regulatory tool that controls the concentration of key signalling proteins, such as those involved in cell cycle control, as well as removing misfolded, damaged or mutant proteins that could be harmful to the cell. Several ubiquitin-like molecules have been discovered, such as Ufm1 (), SUMO1 (), NEDD8, Rad23 (), Elongin B and Parkin (), the latter being involved in Parkinson's disease []. Short Name:  RNF8
Paste the following link

0 Child Features

5 Contains

DB identifier Type Name
IPR013083 Domain Zinc finger, RING/FYVE/PHD-type
IPR001841 Domain Zinc finger, RING-type
IPR000253 Domain Forkhead-associated (FHA) domain
IPR018957 Domain Zinc finger, C3HC4 RING-type
IPR017907 Conserved_site Zinc finger, RING-type, conserved site

1 Cross References

Identifier
PIRSF037950

0 Found In

5 GO Annotations

GO Term Gene Name
GO:0004842 IPR017335
GO:0006302 IPR017335
GO:0006974 IPR017335
GO:0016567 IPR017335
GO:0005634 IPR017335

5 Ontology Annotations

GO Term Gene Name
GO:0004842 IPR017335
GO:0006302 IPR017335
GO:0006974 IPR017335
GO:0016567 IPR017335
GO:0005634 IPR017335

0 Parent Features

0 Proteins

6 Publications

First Author Title Year Journal Volume Pages PubMed ID
            12646216
            15564047
            18793138
            18202552
            16488448
            14976165