Protein Domain : IPR004526

Type:  Family Name:  Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic
Description:  This entry are mostly eukaryotic cytosolic and archaeal forms of the glutamyl-tRNA synthetase. The glutamyl-tRNA synthetases of the eukaryotic cytosol and of the Archaea are more similar to glutaminyl-tRNA synthetases than to bacterial glutamyl-tRNA synthetases. In many species, the charging of tRNA (gln) proceeds first through misacylation with Glu and then transamidation. For this reason, glutamyl-tRNA synthetases, including all known archaeal enzymes may act on both tRNA (gln) and tRNA (glu).Glutamate-tRNA ligase (also known as glutamyl-tRNA synthetase)() is a class Ic ligase and shows several similarities with glutamate-tRNA ligase concerning structure and catalytic properties. It is an alpha2 dimer. To date one crystal structure of a glutamate-tRNA ligase (Thermus thermophilus) has been solved. The molecule has the form of a bent cylinder and consists of four domains. The N-terminal half (domains 1 and 2) contains the 'Rossman fold' typical for class I ligases and resembles the corresponding part of Escherichia coliGlnRS, whereas the C-terminal half exhibits a GluRS-specific structure [].The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Glu-tRNA-synth_arc/euk
Paste the following link

1 Child Features

DB identifier Type Name
IPR022888 Family Glutamyl-tRNA synthetase, archaea

8 Contains

DB identifier Type Name
IPR010987 Domain Glutathione S-transferase, C-terminal-like
IPR014729 Domain Rossmann-like alpha/beta/alpha sandwich fold
IPR011035 Domain Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain
IPR020058 Domain Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
IPR020059 Domain Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain
IPR020056 Domain Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domain
IPR020061 Domain Glutamyl/glutaminyl-tRNA synthetase, class Ib, alpha-bundle domain
IPR001412 Conserved_site Aminoacyl-tRNA synthetase, class I, conserved site

1 Cross References

Identifier
TIGR00463

0 Found In

5 GO Annotations

GO Term Gene Name
GO:0000166 IPR004526
GO:0004818 IPR004526
GO:0005524 IPR004526
GO:0006424 IPR004526
GO:0005737 IPR004526

5 Ontology Annotations

GO Term Gene Name
GO:0000166 IPR004526
GO:0004818 IPR004526
GO:0005524 IPR004526
GO:0006424 IPR004526
GO:0005737 IPR004526

1 Parent Features

DB identifier Type Name
IPR000924 Family Glutamyl/glutaminyl-tRNA synthetase

607 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
121308 D8SNX9 PAC:15416746 Selaginella moellendorffii 731  
evm.model.supercontig_248.3 PAC:16414659 Carica papaya 729  
30128.m008996 B9RFS6 PAC:16818580 Ricinus communis 728  
Cucsa.051070.1 A0A0A0LYW4 PAC:16954173 Cucumis sativus 729  
orange1.1g043341m A0A067DV09 PAC:18107196 Citrus sinensis 758  
AT5G26710.1 O82462 PAC:19670447 Arabidopsis thaliana 719  
Thhalv10003717m V4KQB9 PAC:20198437 Eutrema salsugineum 724  
Ciclev10000427m V4SB37 PAC:20789422 Citrus clementina 721  
Lus10014335 PAC:23164745 Linum usitatissimum 713  
Lus10026044 PAC:23173169 Linum usitatissimum 713  
Potri.013G000400.1 A0A2K1XYM2 PAC:26994360 Populus trichocarpa 732  
Potri.013G000400.2 PAC:26994361 Populus trichocarpa 725  
Potri.005G001200.1 B9N401 PAC:27031313 Populus trichocarpa 728  
Gorai.012G023300.3 A0A0D2V0P6 PAC:26826639 Gossypium raimondii 727  
Gorai.012G023300.1 A0A0D2V0P6 PAC:26826637 Gossypium raimondii 727  
Gorai.012G023300.4 A0A0D2TH84 PAC:26826640 Gossypium raimondii 658  
Gorai.012G023300.2 A0A0D2V0P6 PAC:26826638 Gossypium raimondii 727  
Gorai.011G225600.1 A0A0D2TC21 PAC:26807065 Gossypium raimondii 728  
Gorai.011G225600.2 A0A0D2UWM8 PAC:26807066 Gossypium raimondii 604  
47622 I0YW46 PAC:27388752 Coccomyxa subellipsoidea C-169 831  
64448 C1MKJ2 PAC:27343280 Micromonas pusilla CCMP1545 724  
92936 PAC:27398933 Micromonas sp RCC299 732  
55076 PAC:27399487 Micromonas sp RCC299 717  
39738 A4RRL4 PAC:27417565 Ostreococcus lucimarinus 749  
Thecc1EG047055t1 A0A061F558 PAC:27425319 Theobroma cacao 731  
Migut.B00906.1.p A0A022PYN7 PAC:28946329 Mimulus guttatus 709  
Araha.17843s0001.1.p PAC:28845645 Arabidopsis halleri 602  
Araha.8163s0002.1.p PAC:28850737 Arabidopsis halleri 668  
Cagra.3924s0005.1.p PAC:28900159 Capsella grandiflora 719  
Cagra.6170s0010.1.p PAC:28906156 Capsella grandiflora 718  

7 Publications

First Author Title Year Journal Volume Pages PubMed ID
            8364025
            8274143
            1852601
            2053131
            9426192
            10673435
            2203971