Protein Domain : IPR011068

Type:  Domain Name:  Nucleotidyltransferase, class I, C-terminal-like
Description:  Nucleotidytransferases can be divided into two classes based on highly conserved features of the nucleotidyltransferase motif []. Class I enzymes include eukaryotic poly(A) polymerase (PAP), archaeal tRNA CCA-adding enzyme and possibly DNA polymerase beta, while class II enzymes include eukaryotic and eubacterial tRNA CCA-adding enzymes. This entry represents the C-terminal domain of class I nucleotidyltransferases. The C-terminal domain has an alpha/beta sandwich fold, although the archaeal tRNA CCA-adding enzyme has a large insertion; this fold is reminiscent of the RNA-recognition motif fold. Poly(A) polymerase, the enzyme at the heart of the polyadenylation machinery, is a template-independent RNA polymerase that specifically incorporates ATP at the 3' end of mRNA. In eukaryotes, polyadenylation of pre-mRNA plays an essential role in the initiation step of protein synthesis, as well as in the export and stability of mRNAs. The catalytic domain of poly(A) polymerase shares substantial structural homology with other nucleotidyl transferases such as DNA polymerase beta and kanamycin transferase []. The three invariant aspartates of the catalytic triad ligate two of the three active site metals. One of these metals also contacts the adenine ring. Furthermore, conserved, catalytically important residues contact the nucleotide. These contacts, taken together with metal coordination of the adenine base, provide a structural basis for ATP selection by poly(A) polymerase.The archaeal CCA-adding enzyme builds and repairs the 3 ' end of tRNA. A single active site (nucleotidyltransferase motif) adds both CTP and ATP []. This enzyme is the only RNA polymerase that can build or rebuild a specific nucleic acid sequence without using a nucleic acid template. Short Name:  NuclTrfase_I_C

1 Child Features

DB identifier Type Name
IPR007010 Domain Poly(A) polymerase, RNA-binding domain

0 Contains

1 Cross References

Identifier
SSF55003

2 Found Ins

DB identifier Type Name
IPR014492 Family Poly(A) polymerase
IPR008229 Family tRNA nucleotidyltransferase, archaea

3 GO Annotations

GO Term Gene Name
GO:0003723 IPR011068
GO:0016779 IPR011068
GO:0031123 IPR011068

3 Ontology Annotations

GO Term Gene Name
GO:0003723 IPR011068
GO:0016779 IPR011068
GO:0031123 IPR011068

0 Parent Features

3256 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
231918 D8RMK3 PAC:15420545 Selaginella moellendorffii 548  
169728 D8RAS9 PAC:15404977 Selaginella moellendorffii 534  
evm.model.supercontig_25.22 PAC:16414814 Carica papaya 735  
evm.model.supercontig_3.454 PAC:16416762 Carica papaya 760  
evm.model.supercontig_513.3 PAC:16421930 Carica papaya 608  
evm.model.supercontig_513.4 PAC:16421931 Carica papaya 662  
evm.model.supercontig_9.23 PAC:16428124 Carica papaya 570  
29747.m001069 B9SFV5 PAC:16807735 Ricinus communis 770  
29794.m003414 B9RS15 PAC:16808773 Ricinus communis 754  
29889.m003331 B9RZU0 PAC:16812277 Ricinus communis 492  
30146.m003500 B9RQD8 PAC:16819622 Ricinus communis 712  
Cucsa.121770.1 A0A0A0LQF9 PAC:16961347 Cucumis sativus 748  
Cucsa.161040.1 A0A0A0LP30 PAC:16965448 Cucumis sativus 761  
Cucsa.161090.6 PAC:16965453 Cucumis sativus 720  
Cucsa.161050.2 A0A0A0LP30 PAC:16965449 Cucumis sativus 761  
Cucsa.161100.7 PAC:16965454 Cucumis sativus 720  
Cucsa.161060.3 PAC:16965450 Cucumis sativus 720  
Cucsa.360980.1 PAC:16980828 Cucumis sativus 740  
orange1.1g006658m A0A067GPQ6 PAC:18138637 Citrus sinensis 636  
orange1.1g013399m A0A067FXB5 PAC:18109751 Citrus sinensis 444  
orange1.1g008301m A0A067G901 PAC:18109750 Citrus sinensis 570  
orange1.1g005049m A0A067DUD2 PAC:18121353 Citrus sinensis 716  
orange1.1g005445m A0A067DI15 PAC:18121355 Citrus sinensis 696  
orange1.1g006232m A0A067DIF0 PAC:18121356 Citrus sinensis 655  
orange1.1g004767m A0A067DVM3 PAC:18121352 Citrus sinensis 732  
orange1.1g007342m A0A067DI20 PAC:18121357 Citrus sinensis 607  
orange1.1g005391m A0A067DM47 PAC:18121354 Citrus sinensis 698  
orange1.1g013882m A0A067E9E5 PAC:18116871 Citrus sinensis 434  
orange1.1g010265m A0A067E9S0 PAC:18116870 Citrus sinensis 514  
orange1.1g005418m PAC:18116864 Citrus sinensis 697  

3 Publications

First Author Title Year Journal Volume Pages PubMed ID
            10944102
            8809016
            15590678