Protein Domain : IPR007010

Type:  Domain Name:  Poly(A) polymerase, RNA-binding domain
Description:  In eukaryotes, polyadenylation of pre-mRNA plays an essential role in the initiation step of protein synthesis, as well as in the export and stability of mRNAs. Poly(A) polymerase, the enzyme at the heart of the polyadenylation machinery, is a template-independent RNA polymerase that specifically incorporates ATP at the 3' end of mRNA. The crystal structure of bovine poly(A) polymerase bound to an ATP analogue at 2.5 A resolution has been determined []. The structure revealed expected and unexpected similarities to other proteins. As expected, the catalytic domain of poly(A) polymerase shares substantial structural homology with other nucleotidyl transferases such as DNA polymerase beta and kanamycin transferase. The C-terminal domain unexpectedly folds into a compact domain reminiscent of the RNA-recognition motif fold. The three invariant aspartates of the catalytic triad ligate two of the three active site metals. One of these metals also contacts the adenine ring. Furthermore, conserved, catalytically important residues contact the nucleotide. These contacts, taken together with metal coordination of the adenine base, provide a structural basis for ATP selection by poly(A) polymerase. Short Name:  PolA_pol_RNA-bd_dom

0 Child Features

0 Contains

2 Cross Referencess

Identifier
PF04926
G3DSA:3.30.70.590

1 Found In

DB identifier Type Name
IPR014492 Family Poly(A) polymerase

2 GO Annotations

GO Term Gene Name
GO:0003723 IPR007010
GO:0043631 IPR007010

2 Ontology Annotations

GO Term Gene Name
GO:0003723 IPR007010
GO:0043631 IPR007010

1 Parent Features

DB identifier Type Name
IPR011068 Domain Nucleotidyltransferase, class I, C-terminal-like

3256 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
231918 D8RMK3 PAC:15420545 Selaginella moellendorffii 548  
405615 D8QZ55 PAC:15414687 Selaginella moellendorffii 568  
405613 D8QZ52 PAC:15414685 Selaginella moellendorffii 580  
169728 D8RAS9 PAC:15404977 Selaginella moellendorffii 534  
evm.model.supercontig_25.22 PAC:16414814 Carica papaya 735  
evm.model.supercontig_3.454 PAC:16416762 Carica papaya 760  
evm.model.supercontig_513.3 PAC:16421930 Carica papaya 608  
evm.model.supercontig_513.4 PAC:16421931 Carica papaya 662  
evm.model.supercontig_59.33 PAC:16423170 Carica papaya 198  
evm.model.supercontig_64.34 PAC:16424266 Carica papaya 745  
evm.model.supercontig_9.23 PAC:16428124 Carica papaya 570  
29747.m001069 B9SFV5 PAC:16807735 Ricinus communis 770  
29794.m003414 B9RS15 PAC:16808773 Ricinus communis 754  
29889.m003331 B9RZU0 PAC:16812277 Ricinus communis 492  
30146.m003500 B9RQD8 PAC:16819622 Ricinus communis 712  
30170.m013977 B9R7N2 PAC:16821821 Ricinus communis 889  
Cucsa.121770.1 A0A0A0LQF9 PAC:16961347 Cucumis sativus 748  
Cucsa.161040.1 A0A0A0LP30 PAC:16965448 Cucumis sativus 761  
Cucsa.161090.6 PAC:16965453 Cucumis sativus 720  
Cucsa.161050.2 A0A0A0LP30 PAC:16965449 Cucumis sativus 761  
Cucsa.161100.7 PAC:16965454 Cucumis sativus 720  
Cucsa.161060.3 PAC:16965450 Cucumis sativus 720  
Cucsa.360980.1 PAC:16980828 Cucumis sativus 740  
orange1.1g038251m A0A067D228 PAC:18126814 Citrus sinensis 223  
orange1.1g006658m A0A067GPQ6 PAC:18138637 Citrus sinensis 636  
orange1.1g013399m A0A067FXB5 PAC:18109751 Citrus sinensis 444  
orange1.1g008301m A0A067G901 PAC:18109750 Citrus sinensis 570  
orange1.1g005049m A0A067DUD2 PAC:18121353 Citrus sinensis 716  
orange1.1g005445m A0A067DI15 PAC:18121355 Citrus sinensis 696  
orange1.1g006232m A0A067DIF0 PAC:18121356 Citrus sinensis 655  

1 Publications

First Author Title Year Journal Volume Pages PubMed ID
            10944102