| Type: | Family | Name: | Methionine repressor MetJ |
| Description: | Binding of a specific DNA fragment and S-adenosyl methionine (SAM) co-repressor molecules to the Escherichia colimethionine repressor (MetJ) leads to a significant reduction in dynamic flexibility of the ternary complex, with considerable entropy-enthalpy compensation, not necessarily involving any overall conformational change []. MetJ is a regulatory protein which when combined withS-adenosylmethionine (SAM) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis. It is also autoregulated.The crystal structure of the met repressor-operator complex shows two dimeric repressor molecules bound to adjacent sites 8 base pairs apart on an 18-base-pairDNA fragment. Sequence specificity is achieved by insertion of double-stranded antiparallel protein beta-ribbons into the major groove of B-form DNA, with directhydrogen-bonding between amino-acid side chains and the base pairs. The repressor also recognises sequence-dependent distortion or flexibility of the operatorphosphate backbone, conferring specificity even for inaccessible base pairs []. | Short Name: | Met_repressor_MetJ |
| GO Term | Gene Name |
|---|---|
| GO:0003700 | IPR002084 |
| GO:0006355 | IPR002084 |
| GO:0006555 | IPR002084 |
| DB identifier | UniProt Accession | Secondary Identifier | Organism Name | Length |
|---|---|---|---|---|
| Brdisv1BdTR12c1034897m.p | PAC:34407878 | Brachypodium distachyon BdTR12c |
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