Protein Domain : IPR001818

Type:  Domain Name:  Peptidase M10, metallopeptidase
Description:  Metalloproteases are the most diverse of the four main types of protease, with more than 50 families identified to date. In these enzymes, a divalent cation, usually zinc, activates the water molecule. The metal ion is held in place by amino acid ligands, usually three in number. The known metal ligands are His, Glu, Asp or Lys and at least one other residue is required for catalysis, which may play an electrophillic role. Of the known metalloproteases, around half contain an HEXXH motif, which has been shown in crystallographic studies to form part of the metal-binding site []. The HEXXH motif is relatively common, but can be more stringently defined for metalloproteases as 'abXHEbbHbc', where 'a' is most often valine or threonine and forms part of the S1' subsite in thermolysin and neprilysin, 'b' is an uncharged residue, and 'c' a hydrophobic residue. Proline is never found in this site, possibly because it would break the helical structure adopted by this motif in metalloproteases [].This group of metallopeptidases belong to the MEROPS peptidase family M10 (clan MA(M)). The protein fold of the peptidase domain for members of this family resembles that of thermolysin, the type example for clan MA.Sequences having this domain are extracellular metalloproteases, such as collagenase and stromelysin, which degrade the extracellular matrix, are known as matrixins. They are zinc-dependent,calcium-activated proteases synthesised as inactive precursors (zymogens), which are proteolytically cleaved to yield the active enzyme[, ]. All matrixins and related proteins possess 2 domains: an N-terminaldomain, and a zinc-binding active site domain. The N-terminal domain peptide, cleaved during the activation step, includes a conserved PRCGVPDVoctapeptide, known as the cysteine switch, whose Cys residue chelates the active site zinc atom, rendering the enzyme inactive [, ]. The active enzymedegrades components of the extracellular matrix, playing a role in the initial steps of tissue remodelling during morphogenesis, wound healing,angiogenesis and tumour invasion [, ]. Short Name:  Pept_M10_metallopeptidase

0 Child Features

0 Contains

1 Cross References

Identifier
PF00413

4 Found Ins

DB identifier Type Name
IPR021190 Family Peptidase M10A
IPR016293 Family Peptidase M10A, stromelysin-type
IPR016294 Family Peptidase M10B
IPR001843 Family Fragilysin

4 GO Annotations

GO Term Gene Name
GO:0004222 IPR001818
GO:0008270 IPR001818
GO:0006508 IPR001818
GO:0031012 IPR001818

4 Ontology Annotations

GO Term Gene Name
GO:0004222 IPR001818
GO:0008270 IPR001818
GO:0006508 IPR001818
GO:0031012 IPR001818

1 Parent Features

DB identifier Type Name
IPR006026 Domain Peptidase, metallopeptidase

3256 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
13930 D8RFP7 PAC:15412210 Selaginella moellendorffii 301  
110370 D8S744 PAC:15406730 Selaginella moellendorffii 361  
evm.model.supercontig_29.169 PAC:16416198 Carica papaya 368  
evm.model.supercontig_5.334 PAC:16421456 Carica papaya 357  
29736.m002110 B9S2J2 PAC:16807215 Ricinus communis 364  
29822.m003413 B9RUG6 PAC:16809783 Ricinus communis 311  
29822.m003414 B9RUG7 PAC:16809784 Ricinus communis 321  
30174.m008806 B9RD50 PAC:16822451 Ricinus communis 363  
27428.m000110 B9T6S0 PAC:16798283 Ricinus communis 226  
28619.m000044 B9T8P6 PAC:16801278 Ricinus communis 325  
Cucsa.330820.1 PAC:16978216 Cucumis sativus 292  
Cucsa.011110.1 PAC:16951372 Cucumis sativus 295  
Cucsa.158720.1 PAC:16965165 Cucumis sativus 295  
Cucsa.158820.1 PAC:16965175 Cucumis sativus 295  
Cucsa.158710.1 A0A0A0KS47 PAC:16965164 Cucumis sativus 313  
Cucsa.158730.1 PAC:16965166 Cucumis sativus 293  
Cucsa.158740.1 A0A0A0KU08 PAC:16965167 Cucumis sativus 316  
Cucsa.158750.1 A0A0A0KS39 PAC:16965168 Cucumis sativus 319  
Cucsa.158760.1 PAC:16965169 Cucumis sativus 291  
Cucsa.158770.1 PAC:16965170 Cucumis sativus 267  
Cucsa.158780.1 PAC:16965171 Cucumis sativus 292  
Cucsa.158790.1 PAC:16965172 Cucumis sativus 255  
Cucsa.158800.1 PAC:16965173 Cucumis sativus 264  
Cucsa.158810.1 A0A0A0KVC8 PAC:16965174 Cucumis sativus 303  
Cucsa.356580.1 PAC:16980318 Cucumis sativus 291  
Cucsa.259810.1 PAC:16973078 Cucumis sativus 252  
Cucsa.275230.1 A0A0A0KRY9 PAC:16973958 Cucumis sativus 366  
Cucsa.301780.1 A0A0A0KRA5 PAC:16975655 Cucumis sativus 370  
orange1.1g035681m A0A067DG72 PAC:18130319 Citrus sinensis 320  
orange1.1g039712m A0A067D7U1 PAC:18130318 Citrus sinensis 211  

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            7674922
            2167841
            2841336
            2551898
            1988438